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Broad Institute Inc smart-seq2 rna-seq
Smart Seq2 Rna Seq, supplied by Broad Institute Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/smart-seq2+rna-seq/smart+seq2+rna+seq/pmc11091236-77-15-10
Average 90 stars, based on 1 article reviews
smart-seq2 rna-seq - by Bioz Stars, 2026-09
90/100 stars

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Isolation:

Article Title: APOE4 impairs the microglial response in Alzheimer's disease by inducing TGFβ-mediated checkpoints.
Article Snippet: The APOE4 allele is the strongest genetic risk factor for late-onset Alzheimer’s disease (AD).. The contribution of microglial APOE4 to AD pathogenesis is unknown, although APOE has the most enriched gene expression in neurodegenerative microglia (MGnD).. Here, we show in mice and humans a negative role of microglial APOE4 in the induction of the MGnD response to neurodegeneration.

Article Title: Nasal administration of anti-CD3 mAb (Foralumab) downregulates NKG7 and increases TGFB1 and GIMAP7 expression in T cells in subjects with COVID-19.
Article Snippet: Smart-seq2 RNA-seq was performed by the Broad Genomics platform, Broad Institute of MIT and Harvard.

Article Title: Cardiac endothelial cells maintain open chromatin and expression of cardiomyocyte myofibrillar genes
Article Snippet: In the mouse, in order to identify gene sets representative of endothelial, cardiomyocyte, or fibroblast cells within the heart, gene counts from SMART-Seq2 RNA-Seq were downloaded from Tabula Muris ( https://figshare.com/articles/dataset/Single-cell_RNA-seq_data_from_Smart-seq2_sequencing_of_FACS_sorted_cells_v2_/5829687 ).

Article Title: Cardiac endothelial cells maintain open chromatin and expression of cardiomyocyte myofibrillar genes
Article Snippet: In the mouse, in order to identify gene sets representative of endothelial, cardiomyocyte, or fibroblast cells within the heart, gene counts from SMART-Seq2 RNA-Seq were downloaded from Tabula Muris (https://figshare.com/articles/dataset/Single-cell_RNA-seq_data_from_Smart-seq2_sequenc- ing_of_FACS_sorted_cells_v2_/5829687).

Article Title: RNA-seq characterization of histamine-releasing mast cells as potential therapeutic target of osteoarthritis
Article Snippet: Single-cell Smart-Seq2 RNA-Seq Synovial cells isolated from OA synovial tissue were seeded into 96-well PCR plates and sequenced by the Smart-Seq2 method (Broad Institute, MA).

Article Title: Nasal anti-CD3 mAb (Foralumab) dampens CD3+ T effector function and decreases NKG7 in COVID-19 through a mechanism involving GIMAP-7 and TGFb1
Article Snippet: Smart-seq2 RNA-seq was performed by the Broad Genomics plataform, Broad Institute of MIT and Harvard.

Article Title: Human regulatory memory B cells defined by expression of TIM-1 and TIGIT are dysfunctional in multiple sclerosis
Article Snippet: The plates were stored at -80°C and processed by the BROAD Institute Genomics platform for Smart-Seq2 RNA-seq.

Polymerase Chain Reaction:

Article Title: APOE4 impairs the microglial response in Alzheimer's disease by inducing TGFβ-mediated checkpoints.
Article Snippet: The APOE4 allele is the strongest genetic risk factor for late-onset Alzheimer’s disease (AD).. The contribution of microglial APOE4 to AD pathogenesis is unknown, although APOE has the most enriched gene expression in neurodegenerative microglia (MGnD).. Here, we show in mice and humans a negative role of microglial APOE4 in the induction of the MGnD response to neurodegeneration.

Article Title: Nasal administration of anti-CD3 mAb (Foralumab) downregulates NKG7 and increases TGFB1 and GIMAP7 expression in T cells in subjects with COVID-19.
Article Snippet: Smart-seq2 RNA-seq was performed by the Broad Genomics platform, Broad Institute of MIT and Harvard.

Article Title: Cardiac endothelial cells maintain open chromatin and expression of cardiomyocyte myofibrillar genes
Article Snippet: In the mouse, in order to identify gene sets representative of endothelial, cardiomyocyte, or fibroblast cells within the heart, gene counts from SMART-Seq2 RNA-Seq were downloaded from Tabula Muris ( https://figshare.com/articles/dataset/Single-cell_RNA-seq_data_from_Smart-seq2_sequencing_of_FACS_sorted_cells_v2_/5829687 ).

Article Title: Cardiac endothelial cells maintain open chromatin and expression of cardiomyocyte myofibrillar genes
Article Snippet: In the mouse, in order to identify gene sets representative of endothelial, cardiomyocyte, or fibroblast cells within the heart, gene counts from SMART-Seq2 RNA-Seq were downloaded from Tabula Muris (https://figshare.com/articles/dataset/Single-cell_RNA-seq_data_from_Smart-seq2_sequenc- ing_of_FACS_sorted_cells_v2_/5829687).

Article Title: RNA-seq characterization of histamine-releasing mast cells as potential therapeutic target of osteoarthritis
Article Snippet: Single-cell Smart-Seq2 RNA-Seq Synovial cells isolated from OA synovial tissue were seeded into 96-well PCR plates and sequenced by the Smart-Seq2 method (Broad Institute, MA).

Article Title: Nasal anti-CD3 mAb (Foralumab) dampens CD3+ T effector function and decreases NKG7 in COVID-19 through a mechanism involving GIMAP-7 and TGFb1
Article Snippet: Smart-seq2 RNA-seq was performed by the Broad Genomics plataform, Broad Institute of MIT and Harvard.

Article Title: Human regulatory memory B cells defined by expression of TIM-1 and TIGIT are dysfunctional in multiple sclerosis
Article Snippet: The plates were stored at -80°C and processed by the BROAD Institute Genomics platform for Smart-Seq2 RNA-seq.



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